My Little Pony: Friendship Is Magic (2010)

S01E24 Owl's Well That Ends Well

Spike encounters an owl that Twilight has taken under her wing to assist with their tasks. Feeling insecure about his position as Twilight's loyal assistant, Spike becomes increasingly envious of the new arrival. As his jealousy mounts, he starts to consider drastic measures to ensure that the owl doesn't replace him in Twilight's heart and work life.